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Mouse Monoclonal to LAT

LAT-1D1 (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-442-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-442-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.

Specificity

The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

Regulatory Status

Immunogen

Bacterially expressed full-length rat LAT

Species Reactivity:

  • Mouse
  • Rat

Negative Species:

  • Human

Applications:

  • Western Blotting
    Recommended dilution: 5-10 μg/ml
    Positive control: mouse spleen
  • Immunoprecipitation
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15. [Abstract] [Full Text]
  • Product Specific References

  • *Tolar P, Tumova M, Draber P: New monoclonal antibodies recognizing the adaptor protein LAT. Folia Biol (Praha). 2001;47(6):215-7. [Abstract]
  • *Volná P, Lebduska P, Dráberová L, Símová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Horejsí V, Malissen M, Dráber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13. [Abstract] [Full Text]
  • *Heneberg P, Lebduska P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806. [Abstract]
  • *Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data


    LAT-1D1
    Fig. 1A Distribution of LAT and NTAL on mouse bone marrow-derived mast cells
    LAT-1D1
    Fig. 1B Distribution of LAT and NTAL on rat basophilic leukemia cells


    Fig. 1. Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets.
    LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; 1A) and RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody (cat. no. 11-487-C100), followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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