categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD105 / Endoglin

MEM-229 (IgG2a)

Product No.	Form	Quantity	Price
11-453-C025	purified	0.025 mg	choose region
11-453-C100	purified	0.1 mg	choose region
1B-453-C025	biotin	0.025 mg	choose region
1B-453-C100	biotin	0.1 mg	choose region
1F-453-T025	FITC	25 tests	choose region
1F-453-T100	FITC	100 tests	choose region
1P-453-T025	PE	25 tests	choose region
1P-453-T100	PE	100 tests	choose region

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Background

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Entrez Gene (human): 9q33-q34.1

Specificity

The antibody MEM-229 recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Immunogen

Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

Species Reactivity: Human Porcine Negative Species:	Applications: Flow Cytometry Recommended dilution: 10 μg/ml Positive control: Kg1 human acute myelogenous leukemia cell line Western Blotting Application note: Non-reducing conditions. Immunohistochemistry (frozen sections) Recommended dilution: 1:200 Application note:acetone fixation Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8. [Abstract] [Full Text] Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85. [Abstract] [Full Text] Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45. [Abstract] Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64. [Abstract] Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9. [Abstract] [Full Text] Product Specific References Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol Res. 2009;58(6):885-93. [Abstract]

Species Reactivity:

Human
Porcine

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 10 μg/ml
Positive control: Kg1 human acute myelogenous leukemia cell line
Western Blotting
Application note: Non-reducing conditions.
Immunohistochemistry (frozen sections)
Recommended dilution: 1:200
Application note:acetone fixation
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8. [Abstract] [Full Text]

*Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85. [Abstract] [Full Text]

*Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45. [Abstract]

*Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64. [Abstract]

*Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9. [Abstract] [Full Text]

Product Specific References

*Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol Res. 2009;58(6):885-93. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG2a Isotype Control

Mouse Monoclonal to CD105 MEM-226 (IgG2a)

Example Data

Fig. 1.

Fig. 1. Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).

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