Mouse IgM Isotype Control
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The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
- Other species Not tested (expected negative)
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
For research use only. Not for drug, diagnostic or other use.
Example of nonspecific mouse IgM (PFR-03; purified, GAM-APC) signal on human peripheral blood; surface staining, 6 μg/ml.
Example of nonspecific mouse IgM (PFR-03) FITC signal on human peripheral blood; surface staining, 1 μg/ml.
Example of nonspecific mouse IgM (PFR-03) PE signal on human peripheral blood; surface staining, 3 μg/ml.