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Mouse IgG2b Isotype Control

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  11-801-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PLRV219, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.

Regulatory Status

Negative Species:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Other species Not tested (expected negative)

Applications:

  • Flow Cytometry
  • Control Experiments
    The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Harald Schwarz, Jan Gornicec, Theresa Neuper, Maria Alejandra Parigiani, Michael Wallner, Albert Duschl, and Jutta Horejs-Hoeck: Biological Activity of Masked Endotoxin. Sci Rep. 2017; 7: 44750. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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