System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse IgG2a Isotype Control

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-458-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-458-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  10-458-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  10-458-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-458-C025 Pacific Blue™ 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  PB-458-C100 Pacific Blue™ 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A4-458-C025 Alexa Fluor® 488 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A4-458-C100 Alexa Fluor® 488 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A6-458-C025 Alexa Fluor® 647 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A6-458-C100 Alexa Fluor® 647 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.

Regulatory Status

Negative Species:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Other species Not tested (expected negative)

Applications:

  • Flow Cytometry
  • Control Experiments
    The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    PPV-04 PB


    Fig. 1. Example of nonspecific mouse IgG2a (PPV-04) Pacific BlueTM signal on human peripheral blood; surface staining, 1 μg/ml.


    PPV-04 AF488


    Fig. 2. Example of nonspecific mouse IgG2a (PPV-04) Alexa Fluor® 488 signal on human peripheral blood; surface staining, 3 μg/ml.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy