categories: Mouse Immunoglobulin Isotype Controls

Mouse IgG2a Isotype Control

Product No.	Form	Quantity	Price
11-458-C025	purified	0.025 mg	choose region
11-458-C100	purified	0.1 mg	choose region
10-458-C025	azide free	0.025 mg	choose region
10-458-C100	azide free	0.1 mg	choose region
1B-458-C025	biotin	0.025 mg	choose region
1B-458-C100	biotin	0.1 mg	choose region
PB-458-C025	Pacific Blue™	0.025 mg	choose region
PB-458-C100	Pacific Blue™	0.1 mg	choose region
1F-458-C025	FITC	0.025 mg	choose region
1F-458-C100	FITC	0.1 mg	choose region
A4-458-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-458-C100	Alexa Fluor® 488	0.1 mg	choose region
1P-458-C025	PE	0.025 mg	choose region
1P-458-C100	PE	0.1 mg	choose region
1A-458-C025	APC	0.025 mg	choose region
1A-458-C100	APC	0.1 mg	choose region
A6-458-C025	Alexa Fluor® 647	0.025 mg	choose region
A6-458-C100	Alexa Fluor® 647	0.1 mg	choose region
PC-458-C025	PerCP	0.025 mg	choose region
PC-458-C100	PerCP	0.1 mg	choose region
A7-458-C025	Alexa Fluor® 700	0.025 mg	choose region
A7-458-C100	Alexa Fluor® 700	0.1 mg	choose region

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Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.

Negative Species: Human Porcine Mouse Rat Other species Not tested (expected negative)	Applications: Flow Cytometry Control Experiments The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	References Unpublished.

Negative Species:

Human
Porcine
Mouse
Rat
Other species Not tested (expected negative)

Applications:

Flow Cytometry
Control Experiments
The antibody is suitable for control experiments when performing cell surface staining as well as intracellular staining.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

Unpublished.

For research use only. Not for drug, diagnostic or other use.

Example Data

Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)

DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Cy5, Cy5.5 and Cy7 conjugates are made and sold under license from GE Healthcare Bio-Sciences Corp. The conjugates are licensed for non-commercial research use only. Not for therapeutic or in vivo applications. Other use needs license from GE Healthcare Bio-Sciences Corp. under U.S. patent Nos. 5 569 587 and 5 627 027 and other patents pending.

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