categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human), Serum & Plasma Proteins (Human), CD Antigens & MHC (Veterinary), Serum & Plasma Proteins (Veterinary)

Mouse Monoclonal to beta2-microglobulin

B2M-02 (IgG1)

Product No.	Form	Quantity	Price
11-456-C025	purified	0.025 mg	choose region
11-456-C100	purified	0.1 mg	choose region
F2-456-C100	F(ab')2 fragment	0.1 mg	choose region
1B-456-C025	biotin	0.025 mg	choose region
1B-456-C100	biotin	0.1 mg	choose region
A4-456-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-456-C100	Alexa Fluor® 488	0.1 mg	choose region
1P-456-C025	PE	0.025 mg	choose region
1P-456-C100	PE	0.1 mg	choose region

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- Manufacture on order

Background

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Entrez Gene (human): 15q21-q22.2

Specificity

The antibody B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Immunogen

Purified isolated human beta2-microglobulin.

Species Reactivity: Human Porcine Negative Species:	Applications: Flow Cytometry Recommended dilution: 1 μg/ml Positive control: PBL cell line Negative control: DAUDI human lymphoma cell line, erythrocytes Western Blotting Recommended dilution: 2-4 μg/ml Positive control: U937 human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 5 μg/ml Positive tissue: liver ELISA Application note: Working dilution should be determinated by investigatore. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. [Abstract] Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6. [Abstract]

Species Reactivity:

Human
Porcine

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 1 μg/ml
Positive control: PBL cell line
Negative control: DAUDI human lymphoma cell line, erythrocytes
Western Blotting
Recommended dilution: 2-4 μg/ml
Positive control: U937 human lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Application note: Both reducing and non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 5 μg/ml
Positive tissue: liver
ELISA
Application note: Working dilution should be determinated by investigatore.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. [Abstract]

*Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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