categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human), Serum & Plasma Proteins (Human), Serum & Plasma Proteins (Veterinary), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to beta2-microglobulin

B2M-02 (IgG1)

Product No.	Form	Quantity	Price
11-456-C025	purified	0.025 mg	choose region
11-456-C100	purified	0.1 mg	choose region
F2-456-C100	F(ab')2 fragment	0.1 mg	choose region
1B-456-C025	biotin	0.025 mg	choose region
1B-456-C100	biotin	0.1 mg	choose region

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Background

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Entrez Gene (human): 15q21-q22.2

Specificity

The antibody B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Immunogen

Purified isolated human beta2-microglobulin.

Species Reactivity: Human Porcine Negative Species:	Applications: Flow Cytometry Recommended dilution: 1 μg/ml Positive control: PBL cell line Negative control: DAUDI human lymphoma cell line, erythrocytes Western Blotting Recommended dilution: 2-4 μg/ml Positive control: U937 human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 5 μg/ml Positive tissue: liver ELISA Application note: Working dilution should be determinated by investigatore. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. [Abstract] Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6. [Abstract]

Species Reactivity:

Human
Porcine

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 1 μg/ml
Positive control: PBL cell line
Negative control: DAUDI human lymphoma cell line, erythrocytes
Western Blotting
Recommended dilution: 2-4 μg/ml
Positive control: U937 human lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Application note: Both reducing and non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 5 μg/ml
Positive tissue: liver
ELISA
Application note: Working dilution should be determinated by investigatore.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. [Abstract]

*Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02.

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