System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD50 / ICAM-3

MEM-04 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  12-452-C025 low endotoxin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  12-452-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order


CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.


The antibody MEM-04 reacts with CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).

Regulatory Status


Leukocytes of a patient suffering from LGL-type leukemia.

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
    Recommended dilution:5 μg/ml
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive tissue: tonsil
  • Functional Application
    The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F. ICAM-3 interacts with LFA-1 and regulates the LFA-1/ICAM-1 cell adhesion pathway. J Cell Biol. 1993 Nov;123(4):1007-16. [Abstract]
  • *Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM-3, the third LFA-1 counterreceptor, is a co-stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov;23(11):2799-806. [Abstract]
  • *Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM-3 and LFA-1-mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep;126(5):1277-86. [Abstract]
  • *Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar;17(3):324-33. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    MEM-04 IHC
    Fig. 1.

    Fig. 1. Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04).
    Commercially tested by LifeSpan BioSciences.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy