categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD50 / ICAM-3

MEM-04 (IgG1)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	12-452-C025	low endotoxin	0.025 mg		choose region
	12-452-C100	low endotoxin	0.1 mg		choose region

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Background

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

Entrez Gene: 19p13.3-p13.2

Specificity

The antibody MEM-04 reacts with CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).

Immunogen

Leukocytes of a patient suffering from LGL-type leukemia.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:5 μg/ml Immunohistochemistry (paraffin sections) Recommended dilution:10 μg/ml Positive tissue: tonsil Functional Application The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1). Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F. ICAM-3 interacts with LFA-1 and regulates the LFA-1/ICAM-1 cell adhesion pathway. J Cell Biol. 1993 Nov;123(4):1007-16. [Abstract] Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM-3, the third LFA-1 counterreceptor, is a co-stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov;23(11):2799-806. [Abstract] Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM-3 and LFA-1-mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep;126(5):1277-86. [Abstract] Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar;17(3):324-33. [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:5 μg/ml
Immunohistochemistry (paraffin sections)
Recommended dilution:10 μg/ml
Positive tissue: tonsil
Functional Application
The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F. ICAM-3 interacts with LFA-1 and regulates the LFA-1/ICAM-1 cell adhesion pathway. J Cell Biol. 1993 Nov;123(4):1007-16. [Abstract]

*Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM-3, the third LFA-1 counterreceptor, is a co-stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov;23(11):2799-806. [Abstract]

*Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM-3 and LFA-1-mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep;126(5):1277-86. [Abstract]

*Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar;17(3):324-33. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04).
Commercially tested by LifeSpan BioSciences.

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