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Mouse Monoclonal to Vimentin

VI-10 (IgM)

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  11-460-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.
Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP).

Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin.
Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.


The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.

Regulatory Status

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Chicken
  • Other not tested

Negative Species:


  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 5 μg/ml
    Positive tissue: skin fibroblast
  • Immunocytochemistry
    Staining technique:
    RBL basophilic leukemia cell line:
    (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C;
    (b) Fix cells directly in methanol for 10 min at -20C or in acetone for 10 min at -20C.
    Incubation: 45 min RT
    Positive control:
    3T3 mouse Swiss albino fibroblast cell line
    RBL rat basophilic cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Draberova E, Zikova M, Draber P: Monoclonal antibody VI-10 specific for vimentin. Folia Biol (Praha). 1999;45(1):35-6. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Recombinant Human Vimentin
  • Example Data

    Fig. 1. Immunofluorescence staining (chicken erythrocytes)

    Fig. 1. Immunofluorescence staining of chicken postnatal erythrocytes.
    Tubulin (red) was stained with anti-alpha-tubulin (TU-01; cat. no. 11-250-C100), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

    Fig. 2. Immunofluorescence staining (rat basophils)

    Fig. 2. Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).

    vimentin wb
    Fig. 3.

    Fig. 3. Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa.
    Lr = lysate (reducing conditions)
    Lnr = lysate (non-reducing conditions)
    IPr = immunoprecipitate (reducing conditions)
    IPnr = immunoprecipitate (non-reducing conditions)

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