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Rabbit Polyclonal to Kinesin

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  11-434-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-434-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Specificity

The polyclonal antibody detects total level of endogenous kinesin protein.

Regulatory Status

Immunogen

stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Other not tested

Negative Species:

Applications:

  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Malcova-Janatova I, Richterova V, Draber P, Hasek J: Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains. Folia Microbiol (Praha). 2004;49(6):665-70. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    kin vet mod kat
    Fig. 1.


    Fig. 1. Detection of kinesin in reduced lysates of human HEK cell line (1) and porcine brain (2) by rabbit polyclonal anti-kinesin. 

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