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Mouse Monoclonal to HLA-G

87G (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-437-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-437-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-437-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-437-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-437-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-437-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-437-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A4-437-C025 Alexa Fluor® 488 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A4-437-C100 Alexa Fluor® 488 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-437-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-437-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
The antibody 87G blocks interaction of HLA-G with inhibitory receptors.

Regulatory Status

Immunogen

HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.

Species Reactivity:

  • Human

Negative Species:

  • Mouse
  • Rat

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
    Positive control: JEG-3 human choriocarcinoma epithelial cell line
  • Immunohistochemistry (frozen sections)
    Recommended dilution: 10 μg/ml
    Incubation: 20 min at 25°C
    Positive tissue: extravillous cytotrophoblast
  • ELISA
    Recommended dilution: 1 μg/ml
    Positive control: JEG-3 human choriocarcinoma epithelial cell line
    Application note: The antibody 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32.
  • Functional Application
    Application note: The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Lee N. et al.: The membrane-bound and soluble forms of HLA-G bind identical sets of endogenous peptides but differ with respect to TAP association. Immunity 3(5), 591(1995). [Abstract]
  • Product Specific References

  • *Riteau B. et al.: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 13(2), 193 (2001). [Abstract] [Full Text]
  • *Sageshima N, Ishitani A, Omura M, Akasaki M, Umekage H, Katabuchi H, Okamura H, Hatake K: Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas. Am J Reprod Immunol. 2003 Mar;49(3):174-82. [Abstract]
  • *Ishitani A. et al.: Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA-E, F and G in Maternal-Placental Immune Recognitions. The Journal of Immunology 171, 1376 (2003). [Abstract] [Full Text]
  • *Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8. [Abstract]
  • *Wiendl H: The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy. Brain. 126(Pt 1), 176 (2003). [Abstract] [Full Text]
  • *Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26. [Abstract]
  • *Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul;27(7):643-8. [Abstract]
  • *Hackmon R, Hallak M, Krup M, Weitzman D, Sheiner E, Kaplan B, Weinstein Y: HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy. Fetal Diagn Ther. 2004 Sep-Oct;19(5):404-9. [Abstract]
  • *Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism? Cancer Res. 2005 Nov 15;65(22):10139-44. [Abstract] [Full Text]
  • *Shobu T, Sageshima N, Tokui H, Omura M, Saito K, Nagatsuka Y, Nakanishi M, Hayashi Y, Hatake K, Ishitani A: The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation. J Reprod Immunol. 2006 Dec;72(1-2):18-32. [Abstract]
  • *LeMaoult J, Caumartin J, Daouya M, Favier B, Le Rond S, Gonzalez A, Carosella ED: Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells. Blood. 2007 Mar 1;109(5):2040-8. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data


    87G


    Fig. 1. Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis.
    Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).
    Blocking antibodies:
    Column 1-2: none
    Column 3: Isotype mouse IgG2a control
    Column 4: anti-human HLA-G (87G) purified
    Column 5: anti-human HLA-G (87G) F(ab´)2 fragment
    Column 6: anti-human HLA-G (MEM-G/9; cat. no. 11-292-C100)



    87G biotin

    Fig. 2. Surface staining of HLA-G transfectants (red) compared with K562 cells (orange) and blank (blue), with anti-HLA-G antibody (87G) biotin / streptavidin-PE.



    1F-437

    Fig. 3. Surface staining of HLA-G transfectants with anti-HLA-G antibody (87G) FITC.

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