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Mouse Monoclonal to HLA-G

01G (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-438-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-438-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-438-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-438-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

Regulatory Status

Immunogen

HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.

Species Reactivity:

  • Human

Negative Species:

  • Mouse
  • Rat

Applications:

  • Flow Cytometry
    Recommended dilution:5-6 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Ishitani A. et al., Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA-E, F and G in Maternal-Placental Immune Recognitions. The Journal of Immunology 171, 1376 (2003). [Abstract] [Full Text]
  • Product Specific References

  • *Real LM, Cabrera T, Collado A, Jimenez P, Garcia A, Ruiz-Cabello F, Garrido F: Expression of HLA G in human tumors is not a frequent event. Int J Cancer. 1999 May 17;81(4):512-8. [Abstract]
  • *Real LM, Cabrera T, Canton J, Oliva R, Ruiz-Cabello F, Garrido F: Looking for HLA-G expression in human tumours. J Reprod Immunol. 1999 Jul;43(2):263-73. [Abstract]
  • *Polakova K, Russ G: Expression of the non-classical HLA-G antigen in tumor cell lines is extremely restricted. Neoplasma. 2000;47(6):342-8. [Abstract]
  • *Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8. [Abstract]
  • *Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul;27(7):643-8. [Abstract]
  • *Wiendl H, Feger U, Mittelbronn M, Jack C, Schreiner B, Stadelmann C, Antel J, Brueck W, Meyermann R, Bar-Or A, Kieseier BC, Weller M: Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity. Brain. 2005 Nov;128(Pt 11):2689-2704. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    01G purified

    Fig. 1. Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.


    01G FITC

    Fig. 2. Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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