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Mouse Monoclonal to HLA-G

2A12 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-432-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-432-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-432-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-432-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody 2A12 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

Regulatory Status

Immunogen

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    excellent for intracellular staining
    Recommended dilution:1-10 μg/ml
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *LeMaoult J, Le Discorde M, Rouas-Freiss N, Moreau P, Menier C, McCluskey J, Carosella ED: Biology and functions of human leukocyte antigen-G in health and sickness. Tissue Antigens. 2003 Oct;62(4):273-84. [Abstract]
  • *White SR, Loisel DA, McConville JF, Stern R, Tu Y, Marroquin BA, Noth I, Ober C: Levels of soluble human leukocyte antigen-G are increased in asthmatic airways. Eur Respir J. 2010 Apr;35(4):925-7. [Abstract] [Full Text]
  • *Larsen MH, Bzorek M, Pass MB, Larsen LG, Nielsen MW, Svendsen SG, Lindhard A, Hviid TV: Human leukocyte antigen-G in the male reproductive system and in seminal plasma. Mol Hum Reprod. 2011 Dec;17(12):727-38. [Abstract]
  • *Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    2A12 pur


    Fig. 1. Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM-APC.


    2A12 FITC


    Fig. 2. Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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