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Mouse Monoclonal to HLA-G

5A6G7 (IgG1)

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  11-431-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-431-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  11-431-M001 purified 1.0 mg yes choose region PDF datasheetHTML datasheet buy
  1B-431-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-431-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A4-431-C025 Alexa Fluor® 488 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A4-431-C100 Alexa Fluor® 488 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-431-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-431-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

Regulatory Status

Immunogen

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Western Blotting
    Positive control: JEG-3 cell lysate
    Application note: reducing conditions, 12% AA SDS-PAGE
  • Flow Cytometry
    Recommended dilution: 2 μg/ml
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: placenta tissue
  • Immunohistochemistry (frozen sections)
    Recommended dilution:10 μg/ml
    Positive tissue: placenta tissue
  • Immunocytochemistry
  • ELISA
    Positive control:
    HeLa/HLA-G5 transfectants cell lysate
    HeLa/HLA-G5 cell supernatant
    Negative control: HeLa cell lysate
    Application note: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100).
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26. [Abstract]
  • Product Specific References

  • *Le Rond S, Le Maoult J, Creput C, Menier C, Deschamps M, Le Friec G, Amiot L, Durrbach A, Dausset J, Carosella ED, Rouas-Freiss N: Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA-G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients. Eur J Immunol. 2004 Mar;34(3):649-60. [Abstract]
  • *Rebmann V, Lemaoult J, Rouas-Freiss N, Carosella ED, Grosse-Wilde H: Report of the Wet Workshop for Quantification of Soluble HLA-G in Essen, 2004. Hum Immunol. 2005 Aug;66(8):853-63. [Abstract]
  • *Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45. [Abstract]
  • *Le Rond S, Gonzalez A, Gonzalez AS, Carosella ED, Rouas-Freiss N: Indoleamine 2,3 dioxygenase and human leucocyte antigen-G inhibit the T-cell alloproliferative response through two independent pathways. Immunology. 2005 Nov; 116(3): 297-307. [Full Text]
  • *Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237. [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    5A6G7


    Fig. 1. Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7).
    Lane 1: M8 cell line transfected with empty vector
    Lane 2: M8 cell line transfected with HLA-G1
    Lane 3: M8 cell line transfected with HLA-G5
    Lane 4: M8 cell line transfected with HLA-G6


    5A6G7 PE


    Fig. 2. Surface staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.



    5A6G7 biotin


    Fig. 3. Surface staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.

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