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Mouse Monoclonal to SOCS3 / Suppressor of cytokine signaling

SO1 (IgG2b)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-406-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-406-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.

Specificity

The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.

Regulatory Status

Immunogen

Full-length SOCS3 protein.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Western Blotting
    Positive control: HeLa human cervix carcinoma cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
    Application note: Reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Yu CR, Mahdi RM, Ebong S, Vistica BP, Gery I, Egwuagu CE: Suppressor of cytokine signaling 3 regulates proliferation and activation of T-helper cells. J Biol Chem. 2003 Aug 8;278(32):29752-9. [Abstract] [Full Text]
  • *Kimura A, Kinjyo I, Matsumura Y, Mori H, Mashima R, Harada M, Chien KR, Yasukawa H, Yoshimura A. SOCS3 is a physiological negative regulator for granulopoiesis and granulocyte colony-stimulating factor receptor signaling. J Biol Chem. 2004 Feb 20;279(8):6905-10. [Abstract] [Full Text]
  • *Zhu BM, Ishida Y, Robinson GW, Pacher-Zavisin M, Yoshimura A, Murphy PM, Hennighausen L. SOCS3 Negatively Regulates the gp130-STAT3 Pathway in Mouse Skin Wound Healing. J Invest Dermatol. 2008 Jan 10 [Abstract]
  • *Elliott J: SOCS3 in Liver Regeneration and Hepatocarcinoma. Mol Interv. 2008 Feb;8(1):19-21. [Abstract]
  • *Miao T, Wu D, Zhang Y, Bo X, Xiao F, Zhang X, Magoulas C, Subang MC, Wang P, Richardson PM: SOCS3 suppresses AP-1 transcriptional activity in neuroblastoma cells through inhibition of c-Jun N-terminal kinase. Mol Cell Neurosci. 2008 Feb;37(2):367-75. [Abstract]
  • Product Specific References

  • *Neuwirt H, Puhr M, Cavarretta IT, Mitterberger M, Hobisch A, Culig Z: Suppressor of cytokine signalling-3 is up-regulated by androgen in prostate cancer cell lines and inhibits androgen-mediated proliferation and secretion. Endocr Relat Cancer. 2007 Dec;14(4):1007-19. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2b Isotype Control
  • Example Data


    SO1
    Fig. 1. Western Blotting


    Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line.
    Lane 1: immunostaining with Isotype mouse IgG2b control (PLRV219; cat. no. 11-801-C100)
    Lane 2: immunostaining with anti-SOCS3 (SO1)

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