Mouse Monoclonal to c-Myc / Cellular myelocytomatosis oncogene antibody

9E10 (IgG1)

Product No.	Form	Quantity	Price
11-433-C025	purified	0.025 mg	choose region
11-433-C100	purified	0.1 mg	choose region
1X-433-C025	Peroxidase	0.025 mg	choose region
1X-433-C100	Peroxidase	0.1 mg	choose region
1F-433-C025	FITC	0.025 mg	choose region
1F-433-C100	FITC	0.1 mg	choose region

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Background

Entrez Gene: 8q24.21

Specificity

The antibody 9E10 may be used to detect the c-Myc tag.
The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.

Immunogen

Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-myc.

Species Reactivity: Human Recognizes fusion proteins in all species Negative Species:	Applications: Flow Cytometry Recommended dilution: 1-5 μg/ml Application note: Membrane permeabilization is required. Immunoprecipitation Recommended dilution: 1-5 μg/ml Application note: not suitable for immunoprecipitation of native c-Myc protein Western Blotting Recommended dilution: 0,5-2 μg/ml Positive control: c-Myc tagged protein Immunohistochemistry (paraffin sections) Recommended dilution: 5-10 μg/ml Positive tissue: perfused brain sections, liver, spleen Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421. Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610. Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77. Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987. Spandidos DA et al. 1987. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res 7:1299-304 . Evan GI et al. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610-6 . Persson H, Hennighausen L, Taub R, DeGrado W, Leder P.: Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984 Aug 17;225(4663):687-93. [Abstract] Product Specific References Siegel J, Brandner G, Hess RD.: Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC. Int J Oncol. 1998 Dec;13(6):1259-62. [Abstract] Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W.: Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 2001 Oct;14(10):803-6. [Abstract] Baggio R, Burgstaller P, Hale SP, Putney AR, Lane M, Lipovsek D, Wright MC, Roberts RW, Liu R, Szostak JW, Wagner RW.: Identification of epitope-like consensus motifs using mRNA display. J Mol Recognit. 2002 May-Jun;15(3):126-34. [Abstract] Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ.: A single-chain antibody/epitope system for functional analysis of protein-protein interactions. Biochemistry. 2002 Oct 22;41(42):12729-38. [Abstract] Wang X, Campoli M, Ko E, Luo W, Ferrone S.: Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies. J Immunol Methods. 2004 Nov;294(1-2):23-35. [Abstract] Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C: The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol. 2008 Aug 11;182(3):603-14. [Abstract] [Full Text]

Species Reactivity:

Human
Recognizes fusion proteins in all species

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 1-5 μg/ml
Application note: Membrane permeabilization is required.
Immunoprecipitation
Recommended dilution: 1-5 μg/ml
Application note: not suitable for immunoprecipitation of native c-Myc protein
Western Blotting
Recommended dilution: 0,5-2 μg/ml
Positive control: c-Myc tagged protein
Immunohistochemistry (paraffin sections)
Recommended dilution: 5-10 μg/ml
Positive tissue: perfused brain sections, liver, spleen

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421.

Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610.

Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77.

Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987.

Spandidos DA et al. 1987. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res 7:1299-304 .

Evan GI et al. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610-6 .

Persson H, Hennighausen L, Taub R, DeGrado W, Leder P.: Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984 Aug 17;225(4663):687-93. [Abstract]

Product Specific References

*Siegel J, Brandner G, Hess RD.: Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC. Int J Oncol. 1998 Dec;13(6):1259-62. [Abstract]

* Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W.: Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng. 2001 Oct;14(10):803-6. [Abstract]

*Baggio R, Burgstaller P, Hale SP, Putney AR, Lane M, Lipovsek D, Wright MC, Roberts RW, Liu R, Szostak JW, Wagner RW.: Identification of epitope-like consensus motifs using mRNA display. J Mol Recognit. 2002 May-Jun;15(3):126-34. [Abstract]

*Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ.: A single-chain antibody/epitope system for functional analysis of protein-protein interactions. Biochemistry. 2002 Oct 22;41(42):12729-38. [Abstract]

*Wang X, Campoli M, Ko E, Luo W, Ferrone S.: Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies. J Immunol Methods. 2004 Nov;294(1-2):23-35. [Abstract]

*Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C: The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol. 2008 Aug 11;182(3):603-14. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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