Mouse Monoclonal to CD54 / ICAM-1
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(ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Raji cells and spleen cells fused with NS1 cells
- Flow Cytometry
Recommended dilution: 1 μg/ml
- Western Blotting
- Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Application Note: Prolonged fixation in buffered formalin can destroy the
epitope. Hight temperature antigen unmasking technique is required
- Immunohistochemistry (frozen sections)
Recommended dilution: 5-10 μg/ml
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Boyd AW, Wawryk SO, Burns GF, Fecondo JV.: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell
contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85(9):3095-9. [Abstract]
*Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO.: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73(7):1896-903. [Abstract]
*Springer TA.: Adhesion receptors of the immune system.
Nature. 1990 Aug 2;346(6283):425-34. [Abstract]
*Ockenhouse CF, Betageri R, Springer TA, Staunton DE.: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus.
Cell. 1992 Jan 10;68(1):63-9. Erratum in: Cell 1992 Mar 6;68(5):following 994. [Abstract]
Product Specific References
*Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar;85(Pt 3):731-8.
[Abstract] [Full Text]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG2b Isotype Control
Fig. 1. Flow Cytometry analysis
Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4
) PE. Total viable cells were used for analysis.