Mouse Monoclonal to DDDDK tag
Technical Information Request Inquiry for Bulk Prices
- In stock! Ready for immediate despatch
- Manufacture on order
The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species.
The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Synthetic peptide: DYKDDDDK conjugated to KLH
- Recognizes fusion proteins in all species
- Western Blotting
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37.
*Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Confocal microscopy of COS-7 transfectants, expressing fusion nuclear protein with Flag epitope.
Fig. 1A Fusion nuclear protein
Fig. 1B Cell nuclei
Fig. 1C Merged figures A + B
Fig. 1. Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)
1B - cell nuclei stained with DAPI (blue)
1C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)