Mouse Monoclonal to DDDDK tag

F-tag-01 (IgG1)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-425-C025	purified	0.025 mg		choose region
	11-425-C100	purified	0.1 mg		choose region

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Specificity

The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species.
The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Species Reactivity: Recognizes fusion proteins in all species Negative Species:	Applications: Immunocytochemistry Western Blotting Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. [Abstract] Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print] [Abstract]

Species Reactivity:

Recognizes fusion proteins in all species

Negative Species:

Applications:

Immunocytochemistry
Western Blotting

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. [Abstract]

*Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print] [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Confocal microscopy of COS-7 transfectants, expressing fusion nuclear protein with Flag epitope.

Detection of fusion nuclear protein with T7-Flag epitope - staining by F-tag-01 antibo

Fig. 1A Fusion nuclear protein

Confocal microscopy of COS-7 transfectants, cell nuclei stained by DAPI

Fig. 1B Cell nuclei

Affirmation of nuclear localization of the fusion protein

Fig. 1C Merged figures A + B

Fig. 1. Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)
1B - cell nuclei stained with DAPI (blue)
1C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

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