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categories: Epitope Tags

Mouse Monoclonal to DDDDK tag

F-tag-01 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-425-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-425-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Specificity

The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species.
The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Regulatory Status

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Species Reactivity:

  • Recognizes fusion proteins in all species

Negative Species:

Applications:

  • Immunocytochemistry
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. [Abstract]
  • *Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print] [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    Confocal microscopy of COS-7 transfectants, expressing fusion nuclear protein with Flag epitope.


    Detection of fusion nuclear protein with T7-Flag epitope  - staining by F-tag-01 antibo
    Fig. 1A Fusion nuclear protein
    Confocal microscopy of COS-7 transfectants, cell nuclei stained by DAPI
    Fig. 1B Cell nuclei
    Affirmation of nuclear localization of the fusion protein
    Fig. 1C Merged figures A + B

















    Fig. 1. Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.
    1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)
    1B - cell nuclei stained with DAPI (blue)
    1C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

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