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Mouse Monoclonal to HLA-Class I

W6/32 (IgG2a)

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Background

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Specificity

The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types.
The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders.
This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

Regulatory Status

Immunogen

Membrane of human tonsil cells

Species Reactivity:

  • Human
  • Non-Human Primates
  • Bovine
  • Feline (Cat)

Negative Species:

  • Rabbit

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
    Application note: Non-reducing conditions.
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • ELISA
  • Functional Application
    The antibody W6/32 is suitable as a positive control for HLA tissue typing.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20.
  • [Abstract]
  • *Brodsky, F.M. et al. (1982): Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166.
  • [Abstract]
  • *Neefjes, J.J. et al. (1986): A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347.
  • [Abstract]
  • *Stern, P. et al. (1987): Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.
  • [Abstract]
  • *Kievits F, Ivanyi P: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct;20(2):115-26.
  • [Abstract]
  • *Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466.
  • [Abstract]
  • *Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May;51(5):567-70.
  • [Abstract]
  • *Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr;49(4):312-20.
  • [Abstract]
  • *Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6. [Abstract]
  • *Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov;64(11):1039-44. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data

    W6/32


    Fig. 1. Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) Alexa Fluor® 488.



    W6/32 PE


    Fig. 2. Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) PE. 


    W6-32 MC

    Fig. 3. Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-HLA-class I (W6/32) Gd155. Gated on DNA.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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