categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to HLA-Class I

W6/32 (IgG2a)

Product No.	Form	Quantity	Price
10-422-C025	azide free	0.025 mg	choose region
10-422-C100	azide free	0.1 mg	choose region
1B-422-C025	biotin	0.025 mg	choose region
1B-422-C100	biotin	0.1 mg	choose region
1F-422-C025	FITC	0.025 mg	choose region
1F-422-C100	FITC	0.1 mg	choose region
A4-422-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-422-C100	Alexa Fluor® 488	0.1 mg	choose region
1P-422-C025	PE	0.025 mg	choose region
1P-422-C100	PE	0.1 mg	choose region
A6-422-C025	Alexa Fluor® 647	0.025 mg	choose region
A6-422-C100	Alexa Fluor® 647	0.1 mg	choose region

- In stock! Ready for immediate despatch

- Manufacture on order

Background

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Entrez Gene (HLA-A; human): 6p21.3
Entrez Gene (HLA-B; human): 6p21.3
Entrez Gene (HLA-C; human): 6p21.3

Specificity

The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types.
The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders.
This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

Immunogen

Membrane of human tonsil cells

Species Reactivity: Human Non-Human Primates Bovine Feline (Cat) Negative Species: Rabbit	Applications: Flow Cytometry Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Immunohistochemistry (frozen sections) ELISA Functional Application The antibody W6/32 is suitable as a positive control for HLA tissue typing. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20. [Abstract] Brodsky, F.M. et al. (1982): Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166. [Abstract] Neefjes, J.J. et al. (1986): A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347. [Abstract] Stern, P. et al. (1987): Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091. [Abstract] Kievits F, Ivanyi P.: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct;20(2):115-26. [Abstract] Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466. [Abstract] Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May;51(5):567-70. [Abstract] Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P.: Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr;49(4):312-20. [Abstract] Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6. [Abstract] Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov;64(11):1039-44. [Abstract] *And many other.

Species Reactivity:

Human
Non-Human Primates
Bovine
Feline (Cat)

Negative Species:

Rabbit

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.
Immunohistochemistry (frozen sections)
ELISA
Functional Application
The antibody W6/32 is suitable as a positive control for HLA tissue typing.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20.

[Abstract]

*Brodsky, F.M. et al. (1982): Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166.

[Abstract]

*Neefjes, J.J. et al. (1986): A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347.

[Abstract]

*Stern, P. et al. (1987): Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.

[Abstract]

*Kievits F, Ivanyi P.: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct;20(2):115-26.

[Abstract]

*Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466.

[Abstract]

*Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May;51(5):567-70.

[Abstract]

*Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P.: Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr;49(4):312-20.

[Abstract]

*Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6. [Abstract]

*Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov;64(11):1039-44. [Abstract]

*And many other.

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG2a Isotype Control

Example Data

Fig. 1.

Fig. 1. Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) Alexa Fluor® 488.

PRODUCTS

Mouse Monoclonal to HLA-Class I

W6/32 (IgG2a)

Background

Specificity

Immunogen

Species Reactivity:

Negative Species:

Applications:

Product Specific References

Related Products

Example Data