Mouse Monoclonal to IgG light chain (bovine)
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The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Fraction of Bovine immunoglobulin.
- Western Blotting
Recommended dilution: 0,5-1 μg/ml, overnight in 4°C
Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min
Strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Reducing conditions. SDS-PAGE (12% separating gel).
- Flow Cytometry
- Immunohistochemistry (frozen sections)
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt). 2006 Oct;25(5):309-12.
For research use only. Not for drug, diagnostic or other use.
Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1
. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).