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Mouse Monoclonal to Ig light chain (bovine)

IVA285-1 (IgG1)

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The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

Regulatory Status


Fraction of Bovine immunoglobulin.

Species Reactivity:

  • Bovine
  • Sheep

Negative Species:


  • Western Blotting
    Recommended dilution: 0,5-1 μg/ml, overnight in 4°C
    Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min
    Application notes:
    Strongly reacts with bovine IgG light chains, weakly reacts with IgM.
    Reducing conditions. SDS-PAGE (12% separating gel).
  • Flow Cytometry
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt). 2006 Oct;25(5):309-12. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Fig. 1.

    Fig. 1. Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
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