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Mouse Monoclonal to PRKAR2A

Hs-36 (IgM)

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Background

PRKAR2A (protein kinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Specificity

The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), previously identified under the general name "intra-acrosomal protein".

Regulatory Status

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Immunocytochemistry
    Recommended dilution: 10 μg/ml
    Staining technique: Membrane permeabilization (acetone) is essential.
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8. [Abstract]
  • Product Specific References

  • *Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53(1):42-9. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data

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