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Mouse Monoclonal to LIME

LIME-10 (IgG2a)

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LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.


The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Regulatory Status


COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Species Reactivity:

  • Human

Negative Species:


  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16. [Abstract]
  • *Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49. [Abstract]
  • Product Specific References

  • *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. [Abstract] [Full Text]
  • *Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

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  • Example Data

    Fig. 1. Western Blotting

    Fig. 1. Western Blotting analysis (reducing conditions) of human LIME using anti-human LIME (LIME-10).
    Lane 1: J77 cell line transfected with LIME
    Lane 2: non-transfected J77 cell line (cells are essentialy devoid of endogenous LIME expression)

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