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Mouse Monoclonal to SLP76

SLP-76/03 (IgG2b)

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SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.


The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.

Regulatory Status


Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag

Species Reactivity:

  • Human
  • Porcine
  • Mouse

Negative Species:


  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: thymus
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Pivniouk VI, Martin TR, Lu-Kuo JM, Katz HR, Oettgen HC, Geha RS: SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells. J Clin Invest. 1999 Jun;103(12):1737-43. [Abstract] [Full Text]
  • *Silverman MA, Shoag J, Wu J, Koretzky GA: Disruption of SLP-76 interaction with Gads inhibits dynamic clustering of SLP-76 and FcepsilonRI signaling in mast cells. Mol Cell Biol. 2006 Mar;26(5):1826-38. [Abstract] [Full Text]
  • *Koretzky GA, Abtahian F, Silverman MA: SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan;6(1):67-78. [Abstract]
  • *Beach D, Gonen R, Bogin Y, Reischl IG, Yablonski D: Dual role of SLP-76 in mediating T cell receptor-induced activation of phospholipase C-gamma1. J Biol Chem. 2007 Feb 2;282(5):2937-46. [Abstract] [Full Text]
  • *Bogin Y, Ainey C, Beach D, Yablonski D: SLP-76 mediates and maintains activation of the Tec family kinase ITK via the T cell antigen receptor-induced association between SLP-76 and ITK. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6638-43. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2b Isotype Control
  • Example Data


    Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03).
    Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line
    Lane 2: JURKAT human peripheral blood T cell leukemia cell line
    Lane 3: RAMOS human Burkitt lymphoma cell line

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