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Rabbit Polyclonal to SLP65 / BLNK

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SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa; B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase C gamma2, as well as Grb2 and Nck adaptor proteins; thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.


The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).

Regulatory Status


A fusion protein representing amino acids 171-356 of human BLNK.

Species Reactivity:

  • Mouse

Negative Species:


  • Western Blotting
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
    Application note: Reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Fu C, Turck CW, Kurosaki T, Chan AC: BLNK: a central linker protein in B cell activation. Immunity. 1998 Jul;9(1):93-103. [Abstract]
  • *Jumaa H, Mitterer M, Reth M, Nielsen PJ: The absence of SLP65 and Btk blocks B cell development at the preB cell receptor-positive stage. Eur J Immunol. 2001 Jul;31(7):2164-9. [Abstract]
  • *Kersseboom R, Middendorp S, Dingjan GM, Dahlenborg K, Reth M, Jumaa H, Hendriks RW: Bruton's tyrosine kinase cooperates with the B cell linker protein SLP-65 as a tumor suppressor in Pre-B cells. J Exp Med. 2003 Jul 7;198(1):91-8. [Abstract] [Full Text]
  • *Kersseboom R, Ta VB, Zijlstra AJ, Middendorp S, Jumaa H, van Loo PF, Hendriks RW: Bruton's tyrosine kinase and SLP-65 regulate pre-B cell differentiation and the induction of Ig light chain gene rearrangement. J Immunol. 2006 Apr 15;176(8):4543-52. [Abstract] [Full Text]
  • *Koretzky GA, Abtahian F, Silverman MA: SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan;6(1):67-78. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Fig. 1. Western Blotting

    Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse SLP65 / BLNK antibody.

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