Mouse Monoclonal to CD358 / DR6 / TNFRSF21

DR-6-04-EC (IgG1)

Product No.	Form	Quantity	Price
11-404-C025	purified	0.025 mg	choose region
11-404-C100	purified	0.1 mg	choose region
1P-404-C025	PE	0.025 mg	choose region
1P-404-C100	PE	0.1 mg	choose region
L6-404-C025	DyLight™ 649	0.025 mg	choose region
L6-404-C100	DyLight™ 649	0.1 mg	choose region

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Background

CD358 / DR6 (Death Receptor-6) is a type I transmembrane protein of the TNF receptor superfamily, expressed in most human tissues and able of inducing apoptosis through its cytoplasmic death domain. Unlike TNFR1 and Fas, DR6 induces apoptosis independently of FADD adaptor. In immune system, DR6 serves as an important regulator for CD4+ T cell proliferation and Th differentiation, and provides also a regulatory mechanism for B cell activation and humoral immune responses.

Entrez Gene: 6p21.1-p12.2

Specificity

The antibody DR-6-04-EC recognizes human DR6 (Death receptor-6), a type I transmembrane protein containing cytoplasmic death domain, widely expressed in most human tissues and cell lines.

Immunogen

A fusion protein representing amino acids 42-335 (extracellular part) of human DR6 linked to the Fc portion of human IgG1 was used as an immunogen.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunoprecipitation Application note: The antibody DR-6-04-EC immunoprecipitates overexpressed DR6 from DR6-transfected HEK/293 cells. Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, Vincenz C, Aggarwal BB, Ni J, Dixit VM: Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 1998 Jul 24;431(3):351-6. [Abstract] Kasof GM, Lu JJ, Liu D, Speer B, Mongan KN, Gomes BC, Lorenzi MV: Tumor necrosis factor-alpha induces the expression of DR6, a member of the TNF receptor family, through activation of NF-kappaB. Oncogene. 2001 Nov 29;20(55):7965-75. [Abstract] [Full Text] Rossi D, Gaidano G: Messengers of cell death: apoptotic signaling in health and disease. Haematologica. 2003 Feb;88(2):212-8. [Abstract] [Full Text] Schmidt CS, Liu J, Zhang T, Song HY, Sandusky G, Mintze K, Benschop RJ, Glasebrook A, Yang DD, Na S: Enhanced B cell expansion, survival, and humoral responses by targeting death receptor 6. J Exp Med. 2003 Jan 6;197(1):51-62. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Application note:
The antibody DR-6-04-EC immunoprecipitates overexpressed DR6 from DR6-transfected HEK/293 cells.
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, Vincenz C, Aggarwal BB, Ni J, Dixit VM: Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 1998 Jul 24;431(3):351-6. [Abstract]

*Kasof GM, Lu JJ, Liu D, Speer B, Mongan KN, Gomes BC, Lorenzi MV: Tumor necrosis factor-alpha induces the expression of DR6, a member of the TNF receptor family, through activation of NF-kappaB. Oncogene. 2001 Nov 29;20(55):7965-75. [Abstract] [Full Text]

*Rossi D, Gaidano G: Messengers of cell death: apoptotic signaling in health and disease. Haematologica. 2003 Feb;88(2):212-8. [Abstract] [Full Text]

*Schmidt CS, Liu J, Zhang T, Song HY, Sandusky G, Mintze K, Benschop RJ, Glasebrook A, Yang DD, Na S: Enhanced B cell expansion, survival, and humoral responses by targeting death receptor 6. J Exp Med. 2003 Jan 6;197(1):51-62. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Flow cytometry analysis of cell surface expression of DR6 in 6 different cell lines using purified anti-DR6 antibody (DR-6-04-EC) (detection by Goat anti-mouse IgG1 PE).

Fig. 2.

Fig. 2. Detection of DR6 transiently transfected into HeLa cells (green) using anti-DR6 antibody (DR-6-04-EC). Cell nuclei visualized by DAPI (blue).

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