Mouse Monoclonal to CD261 / TRAIL-R1 / DR4
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(CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL
, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).
The antibody DR-4-02 recognizes TRAIL-R1 (DR4), a human death receptor 4 (468 amino acids) expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.
Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
- Flow Cytometry
Recommended dilution: 3-5 μg/ml
- Functional Application
Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL).
Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells.
Recommended dilution of antibody: 2-3 μg/ml in cultivation medium
Final concentration of TRAIL: 20-200 ng/ml
Application note: It is recommended to add the antibody 15 min before addition of TRAIL.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Corallini F, Milani D, Nicolin V, Secchiero P: TRAIL, caspases and maturation of normal and leukemic myeloid precursors. Leuk Lymphoma. 2006 Aug;47(8):1459-68.
Product Specific References
*Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
For research use only. Not for drug, diagnostic or other use.
Recombinant human TRAIL-R1 (DR4/ TNFRSF10A)/Fc Chimera
Mouse IgG1 Isotype Control
Fig. 1. Flow Cytometry analysis
Flow Cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines.
Cells were stained with purified anti-TRAIL-R1 antibodies followed by Goat anti-mouse IgG PE.
Red - Goat anti-mouse IgG PE (no primary antibody)
Black - anti-TRAIL-R1 comparative antibody
Blue and Green - anti-TRAIL-R1 (DR-4-02
) in duplicate staining
Fig. 2. Confocal microscopy
Immunofluorescence staining (confocal microscopy) of HeLa human cervix carcinoma cell line
transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02