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Mouse Monoclonal to CD261 / TRAIL-R1 / DR4

DR-4-02 (IgG1)

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Background

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Specificity

The antibody DR-4-02 recognizes TRAIL-R1 (DR4), a human death receptor 4 (468 amino acids) expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.

Regulatory Status

Immunogen

Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 3-5 μg/ml
  • Immunoprecipitation
  • Immunocytochemistry
  • Functional Application
    Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL).
    Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells.
    Recommended dilution of antibody: 2-3 μg/ml in cultivation medium
    Final concentration of TRAIL: 20-200 ng/ml
    Application note: It is recommended to add the antibody 15 min before addition of TRAIL.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Corallini F, Milani D, Nicolin V, Secchiero P: TRAIL, caspases and maturation of normal and leukemic myeloid precursors. Leuk Lymphoma. 2006 Aug;47(8):1459-68. [Abstract]
  • Product Specific References

  • *Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Recombinant human TRAIL-R1 (DR4/ TNFRSF10A)/Fc Chimera
  • Mouse IgG1 Isotype Control
  • Example Data


    DR-4-02
    Fig. 1. Flow Cytometry analysis

    Fig. 1. Flow Cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines.
    Cells were stained with purified anti-TRAIL-R1 antibodies followed by Goat anti-mouse IgG PE.



    Histograms:
    Red - Goat anti-mouse IgG PE (no primary antibody)
    Black - anti-TRAIL-R1 comparative antibody
    Blue and Green - anti-TRAIL-R1 (DR-4-02) in duplicate staining


    DR-4-02
    Fig. 2. Confocal microscopy

    Fig. 2. Immunofluorescence staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02).








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