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Mouse Monoclonal to STAT1

SM2 (IgG1)

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  11-405-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-405-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Specificity

The antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Regulatory Status

Immunogen

STAT1 peptide sequence 8-23 (QLDSKFLEQVHQLYD) conjugated to KLH.

Species Reactivity:

  • Human
  • Other not tested

Negative Species:

Applications:

  • Immunoprecipitation
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T.: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13956-61. [Abstract] [Full Text]
  • *Frank D.A.: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56. [Abstract] [Full Text]
  • *Bromberg J, Darnell J.E. Jr: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000 May 15;19(21):2468-73. [Abstract] [Full Text]
  • *Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A.: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31 [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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