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Mouse Monoclonal to CD40

HI40a (IgG1)

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Background

CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.

Specificity

The antibody HI40a recognizes CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.

Regulatory Status

Immunogen

Human CD40a

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    The purified antibody is effective for indirect immunofluorescence staining of human cells by flow cytometry analysis using 1 μg/ml.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions. J Exp Med. 1996 May 1;183(5):2209-18. [Abstract]
  • *Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106. [Abstract]
  • *Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13(3):273-83. [Abstract]
  • *Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    HI40a
    Fig. 1. Flow Cytometry analysis


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD40 (HI40a) FITC. Cells in the lymphocyte gate were used for analysis.

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