System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD40

HI40a (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  10-416-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  10-416-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-416-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-416-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-416-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-416-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order


CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.


The antibody HI40a recognizes CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.

Regulatory Status


Human CD40a

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
    The purified antibody is effective for indirect immunofluorescence staining of human cells by flow cytometry analysis using 1 μg/ml.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions. J Exp Med. 1996 May 1;183(5):2209-18. [Abstract]
  • *Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106. [Abstract]
  • *Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13(3):273-83. [Abstract]
  • *Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14 [Abstract]
  • Product Specific References

  • *Súkeníková L, Černý V, Novotná O, Petrásková P, Boráková K, Kolářová L, Prokešová L, Hrdý J: Different capacity of in vitro generated myeloid dendritic cells of newborns of healthy and allergic mothers to respond to probiotic strain E. coli O83:K24:H31. Immunol Lett. 2017 May 26. pii: S0165-2478(17)30110-4. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Fig. 1. Flow Cytometry analysis

    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD40 (HI40a) FITC. Cells in the lymphocyte gate were used for analysis.

    HI40a PE

    Fig. 2. Surface staining of human peripheral blood cells with anti-human CD40 (HI40a) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy