Mouse Monoclonal to CD235a / Glycophorin A
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(Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
The antibody HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
HLDA VII; WS Code 70299
Synthetic peptide (Human, N-terminal)
- Flow Cytometry
This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 μg per million cells.
- Immunohistochemistry (frozen sections)
The antibody HIR2 agglutinates untreated RBCs but failes to agglutinate papain-treated cells.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401. [Abstract]
*Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597. [Abstract]
Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990.
Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994.
*Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77.
Product Specific References
*Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002); p.577-582.
For research use only. Not for drug, diagnostic or other use.
Mouse IgG2b Isotype Control
Surface staining of human peripheral blood with anti-CD235a (HIR2