categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human)

Mouse Monoclonal to HLA-E

MEM-E/07 (IgG1)

Product No.	Form	Quantity	Price
11-397-C025	purified	0.025 mg	choose region
11-397-C100	purified	0.1 mg	choose region
1B-397-C025	biotin	0.025 mg	choose region
1B-397-C100	biotin	0.1 mg	choose region

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- Manufacture on order

Background

Entrez Gene: 6p21.3

Specificity

The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).

Immunogen

Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunoprecipitation Immunohistochemistry (paraffin sections) Recommended dilution:10 μg/ml Positive tissue: spleen Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003). Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan;66(1):1-12. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Immunohistochemistry (paraffin sections)
Recommended dilution:10 μg/ml
Positive tissue: spleen

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003).

*Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan;66(1):1-12. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).
Commercially tested by LifeSpan BioSciences.

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