categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD13 / Aminopeptidase N

WM15 (IgG1)

Product No.	Form	Quantity	Price
11-396-C025	purified	0.025 mg	choose region
11-396-C100	purified	0.1 mg	choose region
1B-396-C025	biotin	0.025 mg	choose region
1B-396-C100	biotin	0.1 mg	choose region
1F-396-T025	FITC	25 tests	choose region
1F-396-T100	FITC	100 tests	choose region
A4-396-T025	Alexa Fluor® 488	25 tests	choose region
A4-396-T100	Alexa Fluor® 488	100 tests	choose region
1P-396-T025	PE	25 tests	choose region
1P-396-T100	PE	100 tests	choose region
1A-396-T025	APC	25 tests	choose region
1A-396-T100	APC	100 tests	choose region
PC-396-T025	PerCP	25 tests	choose region
PC-396-T100	PerCP	100 tests	choose region
T9-396-T025	PerCP-Cy™5.5	25 tests	choose region
T9-396-T100	PerCP-Cy™5.5	100 tests	choose region

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Background

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Entrez Gene (human): 15q25-q26

Specificity

The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
HLDA III; WS Code M 213
HLDA IV; WS Code M 44
HLDA IV; WS Code M 209
HLDA V; WS Code M MA191

Immunogen

Human AML cells

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Recommended dilution:5 μg/ml Immunoprecipitation Immunohistochemistry (frozen sections) Functional Application The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8. [Abstract] [Full Text] Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50. [Abstract] Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140. [Abstract] [Full Text] Product Specific References Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9. [Abstract] Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20. [Abstract] Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987). Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701. [Abstract] Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555. [Abstract]

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:5 μg/ml
Immunoprecipitation
Immunohistochemistry (frozen sections)
Functional Application
The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8. [Abstract] [Full Text]

*Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50. [Abstract]

*Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140. [Abstract] [Full Text]

Product Specific References

*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9. [Abstract]

*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20. [Abstract]

*Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701. [Abstract]

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Surface staining of human peripheral blood leukocytes with anti-CD13 mouse monoclonal antibody WM15.

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