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Mouse Monoclonal to CD13 / Aminopeptidase N

WM15 (IgG1)

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Background

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Specificity

The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
HLDA III; WS Code M 213
HLDA IV; WS Code M 44
HLDA IV; WS Code M 209
HLDA V; WS Code M MA191

Regulatory Status

Immunogen

Human AML cells

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:5 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Functional Application
    The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8. [Abstract] [Full Text]
  • *Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50. [Abstract]
  • *Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140. [Abstract] [Full Text]
  • Product Specific References

  • *Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9. [Abstract]
  • *Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20. [Abstract]
  • *Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).
  • *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  • *Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701. [Abstract]
  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

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  • Example Data

    WM15


    Fig. 1. Surface staining of human peripheral blood leukocytes with anti-CD13 mouse monoclonal antibody WM15.


    WM15 PE-DyLight 594


    Fig. 2. Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PE-DyLight 594.


    WM15 biotin


    Fig. 3. Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC.


    WM15 PerCP


    Fig. 4. Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.

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