System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to Kinesin (heavy chain)

KN-03 (IgM)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-391-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-391-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order


Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.


The antibody KN-03 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations.
Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments.
The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.

Regulatory Status


Enriched fraction of porcine brain kinesin.

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Other not tested

Negative Species:


  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Macurek L, Draberova E, Richterova V, Bohm KJ, Draber P: Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin. Hybrid Hybridomics. 2002 Dec;21(6):457-62. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data

    Fig. 1. Immunofluorescence staining (rat basophils)

    Fig. 1. Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-03). Nuclei were stained with DAPI (blue).

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy