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Mouse Monoclonal to CD95 / Fas

UT-1 (IgM)

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Background

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

Specificity

The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.

Regulatory Status

Immunogen

HUT-78 human T cell lymphoma cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Functional Application
    The antibody UT-1 induces Fas-mediated apoptosis.
  • Flow Cytometry
    Recommended dilution:2-10 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17(6):1675-87. [Abstract] [Full Text]
  • *Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16. [Abstract] [Full Text]
  • *Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41. [Abstract] [Full Text]
  • *Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63. [Abstract]
  • Product Specific References

  • *Matsuyama W, Mitsuyama H, Ono M, Shirahama Y, Higashimoto I, Osame M, Arimura K: Discoidin domain receptor 1 contributes to eosinophil survival in an NF-kappaB-dependent manner in Churg-Strauss syndrome. Blood. 2007 Jan 1;109(1):22-30. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data


    UT-1


    Fig. 1.
    Induction of apoptosis in various hematopoetic cell lines by use of anti-Fas antibodies.
    Suspensions of used cell lines were incubated with soluble (or without as control) anti-Fas antibodies. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC.
    Histograms:
    Red (full) – untreated control cells
    Black – standard apoptosis-inducing anti-Fas antibody
    Green – anti-Fas (UT-1)

    A – JURKAT human peripheral blood T cell leukemia cell line
    B – TF-1 human bone marrow erythroleukemia cell line
    C – CEM human leukemia cell line
    D – MOLT-4 human acute lymphoblastic T cell leukemia cell line

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