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Mouse Monoclonal to Fyn

FYN-01 (IgG2b)

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  11-375-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-375-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.

Specificity

The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.

Regulatory Status

Immunogen

Bacterially expressed recombinant fragment of human Fyn (aa 7-176).

Species Reactivity:

  • Human
  • Mouse
  • Other not tested

Negative Species:

Applications:

  • Immunoprecipitation
    Positive control: J77 T cell line;
    Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer; lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF)
    Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
  • Western Blotting
    Recommended dilution: 2 μg/ml
    Positive control: JURKAT human T cell leukemia cell line
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 5 μg/ml
    Positive tissue: brain
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Utting O, Priatel JJ, Teh SJ, Teh HS: p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation. J Immunol. 2001 Feb 1;166(3):1540-6. [Abstract] [Full Text]
  • *Talmor-Cohen A, Tomashov-Matar R, Tsai WB, Kinsey WH, Shalgi R: Fyn kinase-tubulin interaction during meiosis of rat eggs. Reproduction. 2004 Oct;128(4):387-93. [Abstract] [Full Text]
  • *Hattori K, Uchino S, Isosaka T, Maekawa M, Iyo M, Sato T, Kohsaka S, Yagi T, Yuasa S: Fyn is required for haloperidol-induced catalepsy in mice. J Biol Chem. 2006 Mar 17;281(11):7129-35. [Abstract] [Full Text]
  • *Wu HY, Hsu FC, Gleichman AJ, Baconguis I, Coulter DA, Lynch DR: Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems. J Biol Chem. 2007 Jul 13;282(28):20075-87. [Abstract] [Full Text]
  • *Filby A, Seddon B, Kleczkowska J, Salmond R, Tomlinson P, Smida M, Lindquist JA, Schraven B, Zamoyska R. Fyn regulates the duration of TCR engagement needed for commitment to effector function. J Immunol. 2007 Oct 1;179(7):4635-44. [Abstract]
  • *Samayawardhena LA, Kapur R, Craig AW: Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells. Blood. 2007 May 1;109(9):3679-86. [Abstract]
  • *Makinodan E, Manabe T, Makinodan M, Yamauchi T, Matsuyoshi H, Sakumura R, Tatsumi K, Wanaka A. A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells. Neuroscience. 2007 Jun 15;147(1):1-4. [Abstract]
  • *Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20. [Abstract]
  • Product Specific References

  • *Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    FYN-01
    Fig. 1.

    Fig. 1. Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01).
    Lane 1: original lysate of T cells
    Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.













    Fyn fibr
    Fig. 2.

    Fig. 2. Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).










    Fyn HeLa
    Fig. 3.

    Fig. 3. Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).










    Fyn myš tr fib
    Fig. 4.

    Fig. 4. Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).















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