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Mouse Monoclonal to Lyn

LYN-01 (IgG1)

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  11-377-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.

Specificity

The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.

Regulatory Status

Immunogen

Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238).

Species Reactivity:

  • Human
  • Mouse
  • Rat

Negative Species:

Applications:

  • Immunoprecipitation
  • Western Blotting
    Recommended dilution:
    1-2 μg/ml, 60 min
    Positive control:
    RBL rat basophilic leukemia cell line
    JURKAT human peripheral blood T cell leukemia cell line
    A-431 human epidermoid carcinoma cell line
    U-937 human histiocytic lymphoma cell line
    Sample preparation:
    Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non-reducing SDS-PAGE sample buffer.
    Application note: Non-reducing conditions. SDS-PAGE (12% separating gel).
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Strzelecka-Kiliszek A, Kwiatkowska K, Sobota A: Lyn and Syk kinases are sequentially engaged in phagocytosis mediated by Fc gamma R. J Immunol. 2002 Dec 15;169(12):6787-94. [Abstract] [Full Text]
  • *Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20. [Abstract]
  • *Davey AM, Krise KM, Sheets ED, Heikal AA: Molecular perspective of antigen-mediated mast cell signaling. J Biol Chem. 2008 Mar 14;283(11):7117-27. [Abstract]
  • *Kinoshita T, Miyano N, Nakai R, Yokota K, Ishiguro H, Tada T: Protein purification and preliminary crystallographic analysis of human Lyn tyrosine kinase. Protein Expr Purif. 2008 Apr;58(2):318-24. [Abstract]
  • *Dos Santos C, Demur C, Bardet V, Prade-Houdellier N, Payrastre B, Récher C: A critical role for Lyn in acute myeloid leukemia. Blood. 2008 Feb 15;111(4):2269-79. [Abstract]
  • Product Specific References

  • *Draberova L, Amoui M, Draber P.: Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology. 1996 Jan;87(1):141-8. [Abstract]
  • *Amoui M, Draberova L, Tolar P, Draber P.: Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur J Immunol. 1997 Jan;27(1):321-8. [Abstract]
  • *Kovarova M, Tolar P, Arudchandran R, Draberova L, Rivera J, Draber P.: Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation. Mol Cell Biol. 2001 Dec;21(24):8318-28. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data




    LYN-01
    Fig. 1.



    Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01).
    Lane 1: negative control
    Lane 2: RBL rat basophilic leukemia cell line
    Lane 3: JURKAT human peripheral blood T cell leukemia cell line
    Lane 4: A-431 human epidermoid carcinoma cell line
    Lane 5: U-937 human histiocytic lymphoma cell line



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