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Mouse Monoclonal to Blood Group Lewis a

7LE (IgG1)

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  11-385-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-385-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Specificity

The antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Regulatory Status

Immunogen

Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).

Species Reactivity:

  • Human
  • Other not tested

Negative Species:

Applications:

  • Immunohistochemistry (paraffin sections)
    Recommended dilution:5 μg/ml
    Positive control:colorectal adenocarcinoma
    Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
  • Western Blotting
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254(1):185-93. [Abstract] [Full Text]
  • *Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J: Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30. [Abstract]
  • Product Specific References

  • *First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems.
  • *Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631-6. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    7LE
    Fig. 1. Immunohistochemistry of paraffin-embedded sections (colorectal adenocarcinoma)



    Fig. 1. Immunohistochemistry staining of colorectal adenocarcinoma (paraffin-embedded sections) with anti-human Blood Group Lewis a (7LE).












    LS 385


    Fig. 2. Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).
    Commercially tested by LifeSpan BioSciences.




    11-385 IHC3


    Fig. 3. Immunohistochemistry staining of human colon adenocarcinoma (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).


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