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Mouse Monoclonal to CD45RB

MEM-143 (IgG1)

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CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.


The antibody MEM-143 reacts with a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.

Regulatory Status


Human peripheral blood lymphocytes.

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
    Recommended dilution:4 μg/ml
    The staining pattern of the MEM-143 show prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive control:human tonsil
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362(1):26-30. [Abstract]
  • *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302. [Abstract] [Full Text]
  • *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65. [Abstract] [Full Text]
  • *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25. [Abstract] [Full Text]
  • *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90. [Abstract]
  • *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19 [Abstract]
  • Product Specific References

  • *Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    MEM-143 IHC
    Fig. 1.

    Fig. 1. Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD45RB (clone MEM-143). Commercially tested by LifeSpan BioSciences.

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