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Mouse Monoclonal to CD16 / FcgammaRIII

MEM-168 (IgM)

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  11-382-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-382-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Specificity

The antibody MEM-168 reacts with CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

Regulatory Status

Immunogen

Human granulocytes

Species Reactivity:

  • Human
  • Non-Human Primates
  • Porcine

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-4 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61. [Abstract] [Full Text]
  • *Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8. [Abstract]
  • *Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8. [Abstract] [Full Text]
  • Product Specific References

  • *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data


    MEM-168
    Fig. 1. Flow Cytometry analysis



    Fig. 1. Surface staining of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC).
    Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. 11-803-C100)
    Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168)
    Cells in the granulocyte gate were used for analysis.

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