Mouse Monoclonal to alpha-Fetoprotein
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The antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
- Immunohistochemistry (paraffin sections)
Recommended dilution:10 μg/ml
Positive tissue: hepatocellular carcinoma
Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB
The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73. [Abstract]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Fig. 1. Immunohistochemistry of paraffin-embedded sections (hepatocellular carcinoma)
Fig. 1. Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).