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Mouse Monoclonal to alpha-Fetoprotein

AFP-11 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-384-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-384-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

Regulatory Status

Immunogen

purified alpha-Fetoprotein

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive tissue: hepatocellular carcinoma
    Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB
  • ELISA
    The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
  • RIA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    AFP-11
    Fig. 1. Immunohistochemistry of paraffin-embedded sections (hepatocellular carcinoma)




    Fig. 1. Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).


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