categories: Adaptor Proteins, Adaptor Proteins (Human), Adaptor Proteins (Rodent), Adaptor Proteins (Veterinary)

Mouse Monoclonal to PAG / Cbp

PAG-C1 (IgG2b)

Product No.	Form	Quantity	Price
11-346-C025	purified	0.025 mg	choose region
11-346-C100	purified	0.1 mg	choose region
11-346-M001	purified	1.0 mg	choose region

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Background

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Gene Locus (human): 8q21.13
Gene Locus (mouse): 3 A1

Specificity

The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Immunogen

C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.

Species Reactivity: Human Mouse Rat Bovine Other not tested Negative Species:	Applications: Immunoprecipitation Positive control: RAJI human Burkitt lymphoma cell line Immunohistochemistry (paraffin sections) Positive tissue:Appendix (germinal center of lymphatic follicle Application note:heat-mediated antigen retrieval in citrate buffer pH 6.1 Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Horejsí V, Zhang W, Schraven B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16. [Abstract] Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA.: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49. [Abstract] Product Specific References Baumgartner M, Angelisova P, Setterblad N, Mooney N, Werling D, Horejsi V, Langsley G.: Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood. 2003 Mar 1;101(5):1874-81. [Abstract] Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A.: Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor. Mol Cell Biol. 2003 Mar;23(6):2017-28. [Abstract] [Full Text] *Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K.: Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem. 2003 May 16;278(20):17597-600. [Abstract] [Full Text]

Species Reactivity:

Human
Mouse
Rat
Bovine
Other not tested

Negative Species:

Applications:

Immunoprecipitation
Positive control: RAJI human Burkitt lymphoma cell line
Immunohistochemistry (paraffin sections)
Positive tissue:Appendix (germinal center of lymphatic follicle
Application note:heat-mediated antigen retrieval in citrate buffer pH 6.1

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Horejsí V, Zhang W, Schraven B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16. [Abstract]

*Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA.: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49. [Abstract]

Product Specific References

*Baumgartner M, Angelisova P, Setterblad N, Mooney N, Werling D, Horejsi V, Langsley G.: Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood. 2003 Mar 1;101(5):1874-81. [Abstract]

*Davidson D, Bakinowski M, Thomas ML, Horejsi V, Veillette A.: Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor. Mol Cell Biol. 2003 Mar;23(6):2017-28. [Abstract] [Full Text]

*Vang T, Abrahamsen H, Myklebust S, Horejsi V, Tasken K.: Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem. 2003 May 16;278(20):17597-600. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse Monoclonal to PAG / Cbp MEM-255 (IgG2a)

Mouse IgG2b Isotype Control

Example Data

Fig. 1. Immunoprecipitation

Fig. 1. Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line.
Western blot was immunostained with anti-human PAG (MEM-255; cat. no. 11-347-C100).
Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1)
Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody; cat. no. 11-409-C100)

Fig. 2.

Fig. 1. Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.

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