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Mouse Monoclonal to NTAL / LAB

NAP-07 (IgG1)

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Background

NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Specificity

The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

Regulatory Status

Immunogen

Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Species Reactivity:

  • Human
  • Mouse
  • Other not tested

Negative Species:

Applications:

  • Flow Cytometry
    Positive tissue:
    RAJI human lymphoma cell line
    RAMOS human lymphoma cell line
  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 2 μg/ml, incubation 60 min
    Positive control:
    RAMOS human lymphoma cell line
    Negative control:
    HeLa human cervix carcinoma cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel.
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T.: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15. [Abstract] [Full Text]
  • Product Specific References

  • *Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V.: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196(12):1617-26. [Abstract] [Full Text]
  • *Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM.: NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood. 2004 Jul 1;104(1):207-14. [Abstract] [Full Text]
  • *Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P.: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13. [Abstract] [Full Text]
  • *Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P.: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Rabbit Polyclonal to NTAL / LAB
  • Rabbit Polyclonal to NTAL / LAB
  • Rabbit Polyclonal to NTAL / LAB
  • Example Data


    NTAL-membrane localization by confocal microscopy
    Fig. 1. Confocal microscopy


    Fig. 1. Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.
    THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).

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