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Mouse Monoclonal to Acrosin (Porcine)

ACR-2 (IgG1)

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The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

Regulatory Status


Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Species Reactivity:

  • Porcine

Negative Species:

  • Human
  • Bovine
  • Canine (Dog)


  • Flow Cytometry
    described in Ded et al., 2010
  • Immunocytochemistry
    Application note: Membrane permeabilization (acetone) is essential.
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *PeknicovŠ J. et al., Anim. Reprod. Sci. 35, 255 (1994).
  • Product Specific References

  • *Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22(5):427-35. [Abstract]
  • *Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294(1-2):27-30. [Abstract]
  • *Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207. [Abstract]
  • *Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49(2):408-15. [Abstract]
  • *Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56(2):211-23. [Abstract]
  • *Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010 Jul 13;8:87 [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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