categories: Fertility & Reproduction, Fertility & Reproduction (Veterinary)

Mouse Monoclonal to Acrosin (Porcine)

ACR-2 (IgG1)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-260-C025	purified	0.025 mg		choose region
	11-260-C100	purified	0.1 mg		choose region

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Background

Entrez Gene: 5p15

Specificity

The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

Immunogen

Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Species Reactivity: Porcine Negative Species: Human Bovine Canine (Dog)	Applications: Immunocytochemistry Application note: Membrane permeabilization (acetone) is essential. Western Blotting Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Peknicová J. et al., Anim. Reprod. Sci. 35, 255 (1994). Product Specific References Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22(5):427-35. [Abstract] Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294(1-2):27-30. [Abstract] Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207. [Abstract] Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49(2):408-15. [Abstract] Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56(2):211-23. [Abstract]

Species Reactivity:

Porcine

Negative Species:

Human
Bovine
Canine (Dog)

Applications:

Immunocytochemistry
Application note: Membrane permeabilization (acetone) is essential.
Western Blotting

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Peknicová J. et al., Anim. Reprod. Sci. 35, 255 (1994).

Product Specific References

*Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22(5):427-35. [Abstract]

*Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294(1-2):27-30. [Abstract]

*Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176(3):199-207. [Abstract]

*Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49(2):408-15. [Abstract]

*Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56(2):211-23. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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