Mouse Monoclonal to VCP
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VCP (valosin-containing protein)
, also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
*Pavlásek J, Peknicová J, Ulcová-Gallová Z, Nováková P, Reischig J, Micanová Z, Rokyta Z: Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction. Ceska Gynekol. 2004 Jul;69(4):306-11.
Product Specific References
*Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
*Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
*Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for
male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53(1):42-9.
For research use only. Not for drug, diagnostic or other use.
Mouse IgM Isotype Control
Immunofluorescence analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody Hs-14
demonstrates its location to the acrosome. (Normal spermiogram shown).