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Mouse Monoclonal to Cytokeratin (Pan-reactive)

C-11 (IgG1)

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  11-108-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1B-108-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-108-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity

The antibody C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Regulatory Status

Immunogen

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Species Reactivity:

  • Mammalian

Negative Species:

Applications:

  • Western Blotting
  • Flow Cytometry
    Recommended dilution:0.5 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. [Abstract]
  • *Vojtĕsek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82. [Abstract]
  • *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. [Abstract]
  • *Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26. [Abstract]
  • *Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    C-11
    Fig. 1. Intracellular Flow Cytometry analysis


    Fig. 1. Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody (C-11) PE.
    Overlay with Isotype mouse IgG1 control (PPV-06; cat. no. 1P-457-C100)

     

     

    C-11 IHC
    Fig. 2.


    Fig. 2. Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

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