System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to Cytokeratin 19

A53-B/A2 (IgG2a)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-120-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-120-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-120-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-120-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-120-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-120-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A4-120-C025 Alexa Fluor® 488 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A4-120-C100 Alexa Fluor® 488 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity

The antibody A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

Regulatory Status

Immunogen

MCF-7 human breast adenocarcinoma cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Immunohistochemistry (paraffin sections)
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol. 1985 Jun;21(6):733-40. [Abstract]
  • *Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry. 1988;89(4):369-77. [Abstract]
  • *Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J. 1986 Oct;18(10):565-75. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data


    A53-B/A2


    Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.
    Lane 1,2: immunostaining with anti-human Cytokeratin 19 (BA-17; cat. no. 11-119-C100)
    Lane 3,4: immunostaining with anti-human Cytokeratin 19 (A53-B/A2)







    A53-B-A2 FITC


    Fig. 2. Intracellular staining of MCF-7 human breast adenocarcinoma cell line with anti-cytokeratin 19 (A53-B/A2) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy