Mouse Monoclonal to Cytokeratin 19
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are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
The antibody A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
MCF-7 human breast adenocarcinoma cell line
- Immunohistochemistry (paraffin sections)
- Flow Cytometry
- Western Blotting
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol. 1985 Jun;21(6):733-40. [Abstract]
*Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye.
*Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J. 1986 Oct;18(10):565-75. [Abstract]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG2a Isotype Control
Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.Lane 1,2:
immunostaining with anti-human Cytokeratin 19 (BA-17; cat. no. 11-119-C100)Lane 3,4:
immunostaining with anti-human Cytokeratin 19 (A53-B/A2
Intracellular staining of MCF-7 human breast adenocarcinoma cell line with anti-cytokeratin 19 (A53-B/A2
Intracellular staining of HT-29 cell line with anti-cytokeratin 19 (A53-B/A2
) Alexa Fluor®
Intracellular staining of MCF-7 human breast adenocarcinoma cell line with anti-cytokeratin 19 (A53-B/A2) biotin.