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Mouse Monoclonal to Cytokeratin 18

DA-7 (IgG1)

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Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Specificity

The antibody DA-7 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Regulatory Status

Immunogen

Human breast carcinoma cell line PMC-42.

Species Reactivity:

  • Human

Negative Species:

  • Porcine
  • Mouse
  • Rat
  • Hamster
  • Bovine
  • Canine (Dog)
  • Sheep

Applications:

  • Immunoprecipitation
  • Western Blotting
    Recommended dilution:
    1-2 μg/ml, overnight in 4°C
    Positive control:
    HeLa human cervix carcinoma cell line
    A-431 human epidermoid carcinoma cell line
    MCF-7 human breast adenocarcinoma cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-reducing conditions. SDS-PAGE (10% separating gel).
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: colon, skin
  • Immunocytochemistry
    Positive control:
    MCF-7 human breast adenocarcinoma cell line
    HeLa human cervix carcinoma cell line
  • ELISA
    Positive control: MCF-7 human breast adenocarcinoma cell line
    Application note: The antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Taylor-Papadimitriou J, Stampfer M, Bartek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13. [Abstract]
  • *Kovarik J. et al.: J. Tumor Marker Oncol. 5, 219 (1990).
  • Product Specific References

  • *Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. [Abstract]
  • *Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. [Abstract]
  • *Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Mouse Monoclonal to Cytokeratin 18 C-04 (IgG1)
  • Mouse Monoclonal to Cytokeratin 18 DC-10 (IgG1)
  • Example Data


    DA-7
    Fig. 1. Western Blotting


    Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate using anti-human Cytokeratin 18 (DA-7).
    Lane 1: HeLa human cervix carcinoma cell line
    Lane 2: A-431 human epidermoid carcinoma cell line
    Lane 3: MCF-7 human breast adenocarcinoma cell line


    DA-7 IHC
    Fig. 2.


    Fig. 2. Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DA-7).
    Commercially tested by LifeSpan BioSciences.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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