categories: Cytoskeleton: Intermediate Filaments, Cytoskeleton: Intermediate Filaments (Human)

Mouse Monoclonal to Cytokeratin 18

DC-10 (IgG1)

Product No.	Form	Quantity	Price
11-107-C025	purified	0.025 mg	choose region
11-107-C100	purified	0.1 mg	choose region
1B-107-C025	biotin	0.025 mg	choose region
1B-107-C100	biotin	0.1 mg	choose region
1F-107-C025	FITC	0.025 mg	choose region
1F-107-C100	FITC	0.1 mg	choose region

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Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Cytokeratin 18 belongs to type I family (acidic cytokeratins).

Entrez Gene (cytokeratin 18, human): 12q13

Specificity

The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Immunogen

Human breast carcinoma cell line PMC-42.

Species Reactivity: Human Negative Species: Porcine Mouse Rat Hamster Bovine Canine (Dog) Sheep	Applications: Immunoprecipitation Western Blotting Recommended dilution: 1-2 μg/ml, overnight in 4°C Positive control: HeLa human cervix carcinoma cell line MCF-7 human breast adenocarcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel). Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive tissue: colon, skin Immunocytochemistry Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line ELISA Positive control: MCF-7 human breast adenocarcinoma cell line Application note: The antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100). Flow Cytometry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. [Abstract] Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. [Abstract] *Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82. [Abstract]

Species Reactivity:

Human

Negative Species:

Porcine
Mouse
Rat
Hamster
Bovine
Canine (Dog)
Sheep

Applications:

Immunoprecipitation
Western Blotting
Recommended dilution:
1-2 μg/ml, overnight in 4°C
Positive control:
HeLa human cervix carcinoma cell line
MCF-7 human breast adenocarcinoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (10% separating gel).
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: colon, skin
Immunocytochemistry
Positive control:
MCF-7 human breast adenocarcinoma cell line
HeLa human cervix carcinoma cell line
ELISA
Positive control: MCF-7 human breast adenocarcinoma cell line
Application note: The antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).
Flow Cytometry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. [Abstract]

*Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. [Abstract]

*Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Mouse Monoclonal to Cytokeratin 18 C-04 (IgG1)

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Mouse Monoclonal to Cytokeratin 18 DA-7 (IgG1)

Example Data

Fig. 1.

Fig. 1. Detection of cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10.

Fig. 2.

Fig. 2. Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).
Commercially tested by LifeSpan BioSciences.

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