categories: Cytoskeleton: Intermediate Filaments, Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Veterinary)

Mouse Monoclonal to Cytokeratin 18

C-04 (IgG1)

Product No.	Form	Quantity	Price
11-106-C025	purified	0.025 mg	choose region
11-106-C100	purified	0.1 mg	choose region
1B-106-C025	biotin	0.025 mg	choose region
1B-106-C100	biotin	0.1 mg	choose region
1F-106-C025	FITC	0.025 mg	choose region
1F-106-C100	FITC	0.1 mg	choose region
A4-106-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-106-C100	Alexa Fluor® 488	0.1 mg	choose region
A7-106-C025	Alexa Fluor® 700	0.025 mg	choose region
A7-106-C100	Alexa Fluor® 700	0.1 mg	choose region

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Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Entrez Gene (cytokeratin 18, human): 12q13

Specificity

The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.

Immunogen

Cytoskeleton preparation of epidermal carcinoma cell line A431.

Species Reactivity: Mammalian Negative Species:	Applications: Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive control: colon, kidney Flow Cytometry Immunoprecipitation Western Blotting Recommended dilution: 1-2 μg/ml, overnight in 4°C Positive control: HeLa human cervix carcinoma cell line MCF-7 human breast adenocarcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel). Immunocytochemistry ELISA Application note: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Taylor-Papadimitriou J, Stampfer M, Bartek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13. [Abstract] Kovarik J. et al., J. Tumor Marker Oncol. 5, 219 (1990). Cap J, Cerman J, Nemecek S, Marekova M, Hana V, Frysak Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10(4):444-8. [Abstract] Product Specific References Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. [Abstract] Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. [Abstract] Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82. [Abstract] *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. [Abstract]

Species Reactivity:

Mammalian

Negative Species:

Applications:

Immunohistochemistry (paraffin sections)
Recommended dilution:
10 μg/ml
Positive control: colon, kidney
Flow Cytometry
Immunoprecipitation
Western Blotting
Recommended dilution:
1-2 μg/ml, overnight in 4°C
Positive control:
HeLa human cervix carcinoma cell line
MCF-7 human breast adenocarcinoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (10% separating gel).
Immunocytochemistry
ELISA
Application note: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100).

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Taylor-Papadimitriou J, Stampfer M, Bartek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13. [Abstract]

*Kovarik J. et al., J. Tumor Marker Oncol. 5, 219 (1990).

*Cap J, Cerman J, Nemecek S, Marekova M, Hana V, Frysak Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10(4):444-8. [Abstract]

Product Specific References

*Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504. [Abstract]

*Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. [Abstract]

*Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82. [Abstract]

*Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse Monoclonal to Cytokeratin 18 DC-10 (IgG1)

Mouse Monoclonal to Cytokeratin 18 DA-7 (IgG1)

Mouse IgG1 Isotype Control

Example Data

Fig. 1. Immunohistochemistry of paraffin-embedded sections (kidney)

Fig. 1. Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Fig. 2. Intracellular Flow Cytometry analysis

Fig. 2. Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04) FITC.
Overlay with Isotype mouse IgG1 control (PPV-06; cat. no. 1F-457-C100).

Fig. 3.

Fig. 3. Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).
Commercially tested by LifeSpan BioSciences.

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