Mouse Monoclonal to Cytokeratin 7+17
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are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene (cytokeratin 7, human): 12q12-q13
Entrez Gene (cytokeratin 17, human): 17q12-q21
The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
- Western Blotting
Recommended dilution: 1 μg/ml
Positive control: HeLa human cell line
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
- Immunohistochemistry (paraffin sections)
Recommended dilution: 20 μg/ml
Pretreatment: 0,1% pepsin, 30 min, 37°C
Incubation: 4°C overnight
Positive tissue: pancreas
Application note: chromogen DAB
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Vojtesek B, Staskova Z, Nenutil R, Bartkova J, Kovarik J, Rejthar A, Bartek J: A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Fig. 1. Immunohistochemistry of paraffin-embedded sections (pancreas)
staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7+17 (C-46
Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46
Immunohistochemistry staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46
Commercially tested by LifeSpan BioSciences.