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Mouse Monoclonal to Cytokeratin 5+18

C-50 (IgG1)

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Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.


The antibody C-50 reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Regulatory Status


Cytoskeleton preparation from HeLa cells.

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Hamster
  • Bovine
  • Canine (Dog)
  • Sheep

Negative Species:

  • Chicken
  • Xenopus
  • Other not tested


  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive control:Human prostate
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Fig. 1. Western Blotting

    Fig. 1. Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.
    Lane 1: MW standard
    Lane 2: immunostaining with anti-cytokeratin 5+18 (clone C-50)
    Lane 3: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 1P-457-C100)

    C-50 IHC
    Fig. 2.

    Fig. 2. Immunohistochemistry staining of human prostate (paraffin section) using anti-cytokeratin 5+18 (clone C-50). Commercially tested by LifeSpan BioSciences.

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