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Mouse Monoclonal to Cytokeratin 5+18

C-50 (IgG1)

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Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity

The antibody C-50 reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Regulatory Status

Immunogen

Cytoskeleton preparation from HeLa cells.

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Hamster
  • Bovine
  • Canine (Dog)
  • Sheep

Negative Species:

  • Chicken
  • Xenopus
  • Other not tested

Applications:

  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive control:Human prostate
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    C-50
    Fig. 1. Western Blotting


    Fig. 1. Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.
    Lane 1: MW standard
    Lane 2: immunostaining with anti-cytokeratin 5+18 (clone C-50)
    Lane 3: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 1P-457-C100)


    C-50 IHC
    Fig. 2.


    Fig. 2. Immunohistochemistry staining of human prostate (paraffin section) using anti-cytokeratin 5+18 (clone C-50). Commercially tested by LifeSpan BioSciences.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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