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Mouse Monoclonal to betaIII-tubulin

TU-20 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-264-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-264-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-264-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-264-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Specificity

The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

Regulatory Status

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Species Reactivity:

  • Broad species reactivity

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
    Recommended dilution:1-2 μg/ml, 90 min
    Positive control: Porcine brain lysate
    Negative control: HPB-ALL human peripheral blood leukemia cell line
    Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
    Application note: Reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Staining technique: Standard ABC technique (DAB+)
    Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
  • Immunocytochemistry
    Positive material: Neuro2a mouse neuroblastoma cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Draberova E, Lukas Z, Ivanyi D, Viklicky V, Draber P: Expression of class III beta-tubulin in normal and neoplastic human tissues. Histochem Cell Biol. 1998 Mar;109(3):231-9. [Abstract]
  • *Peknicova J, Kubatova A, Sulimenko V, Draberova E, Viklicky V, Hozak P, Draber P: Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail. Biol Reprod. 2001 Sep;65(3):672-9. [Abstract] [Full Text]
  • *Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V: Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody. Indian J Med Res. 2009 Jan;129(1):89-94. [Abstract]
  • *Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res. 2004 Aug 1;298(1):218-28. [Abstract]
  • *Jirasek T, Pisarikova E, Viklicky V, Mandys V: Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies. Folia Histochem Cytobiol. 2007;45(1):41-5. [Abstract]
  • *Katsetos CD, Draberova E, Smejkalova B, Reddy G, Bertrand L, de Chadarévian J-P, Legido A, Nissanov J, Baas PW, Draber P: Class III β-Tubulin and γ-Tubulin are Co-expressed and form Complexes in Human Glioblastoma Cells. Neurochem Res (2007) 32:1387-1398.
  • *Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M: A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation. Genes Dev. 2009 Mar 1;23(5):575-88. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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  • Mouse IgG1 Isotype Control
  • Example Data




    TU-20 ICC
    Fig. 1. Immunofluorescence staining (mouse neuroblastoma cells)




    Fig. 1. Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 μg/ml). Nuclei were stained with DAPI (blue).









    TU-20
    Fig. 2A Immunofluorescence staining (mouse embryonal carcinoma cells)
    TU-20
    Fig. 2B Immunofluorescence staining (mouse embryonal carcinoma cells)


    Fig. 2. Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
    2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red).
    2B - Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. 11-251-C100).
    Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).







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